Skip to main content
. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: J Bone Miner Res. 2016 Apr 20;31(9):1652–1665. doi: 10.1002/jbmr.2848

Figure 2.

Figure 2

The BMP signaling pathway is regulated by the cellular response to hypoxia. (A) SMAD 1/5/8 phosphorylation in SHED cells under hypoxia. SMAD 1/5/8 phosphorylation and HIF-1α were detected by Western blot analysis after the indicated number of hours under hypoxia (n = 5). (B) Relative ID1 expression in SHED cells under hypoxia. ID1 levels were quantified by real-time PCR and normalized to 18s RNA (n = 3). (C) Dorsomorphin, but not Noggin or p38 MAPK inhibitor SB203580, mitigates SMAD 1/5/8 phosphorylation in SHED cells under low oxygen conditions. SMAD 1/5/8 phosphorylation was detected by Western blot analysis after two hours under hypoxia. Noggin was used at 400ng/ml (n = 3). (D) ID1 expression induced by hypoxia in SHED cells is inhibited by Dorsomorphin, but not Noggin or p38 MAPK inhibitor SB203580. ID1 levels were quantified by real-time PCR and normalized to 18s RNA (n = 3). C, Unaffected controls; F, FOP. Genotype and hypoxia, as well as genotype and drug inhibition, are significantly related to SMAD 1/5/8 phosphorylation and ID1 expression by 2-way ANOVA (p < 0.0001). Bonferroni post-hoc analysis: ****, p < 0.0001; ***, p < 0.001; **, p < 0.01; *, p < 0.05.