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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: J Bone Miner Res. 2016 Apr 20;31(9):1652–1665. doi: 10.1002/jbmr.2848

Figure 3.

Figure 3

mACVR1 enhances chondrogenesis of SHED cells under hypoxia. (A) Representative chondrocyte pellets and quantification of chondrocyte pellet size under the indicated conditions (n = 6). Scale bar = 1 mm. (B) Histological appearance and quantification of extracellular matrix showing enhanced chondrocyte differentiation (n =3). Scale bar = 100 µm. (C) Up-regulation of chondrocyte markers COL2A1, Sox9, and aggrecan (ACAN) in SHED cells under hypoxic conditions (n = 3). Genotype and hypoxia are significantly related to pellet size and chondrocyte extracellular matrix by 2-way ANOVA (p < 0.01; interaction, p < 0.01). Post-hoc analysis: ****, p < 0.0001; ***, p < 0.001; **, p < 0.01; *, p < 0.05.