Skip to main content
. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Mol Cell. 2016 Aug 18;63(5):865–876. doi: 10.1016/j.molcel.2016.07.010

FIGURE 2. LAF-1 induces ssRNA compaction.

FIGURE 2

(A) FRET DNA constructs containing 5’ U15-50 tail and Cy3/Cy5 dyes across ssRNA. (B) FRET histograms before (gray) and after (light blue) LAF-1 addition. (C) Averaged Cy3 and Cy5 intensities obtained from smFRET experiment. Arrow indicates when LAF-1 was added (D) Representative single molecule FRET traces for U30, 40 and 50. Rapid FRET increase occurs immediately after addition of LAF-1 (20nM). See also Figure S2 and 3.