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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: J Bone Miner Res. 2016 Jun 2;31(9):1688–1700. doi: 10.1002/jbmr.2854

Figure 4.

Figure 4

A PMMA-induced osteolytic lesion has elevated level of MIF and diminished amount of SDF-1 chemokines in vivo. A) and B) Concentrations of MIF detected in calvaria and femur tissues of control (no particles placed) and PMMA particle-induced osteolytic calvarial lesions in WT animals; C) and D) The amounts of SDF-1 in calvaria and femur tissues of control (no particles placed) and PMMA particle-induced osteolytic calvarial lesions in WT and MIF-KO animals. PMMA particles suspended in PBS or PBS alone were placed over calvarial tissues of WT or MIF-KO animals (n=5 animals/group). At day 3, the tissues were dissected and homogenized in PBS buffer containing Tween 20. The supernatants were subjected to a sandwich ELISA. **p<0.01, ***p<0.001.