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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Anal Bioanal Chem. 2016 Jul 15;408(23):6461–6471. doi: 10.1007/s00216-016-9765-8

Table 1.

LC-MS/MS parameters for cannabinoids, their metabolites and internal standards in human urine. Bold masses depict quantifier transition.

Analyte Q1 mass (amu) Q3 mass (amu) Dwell time (ms) Q1 Pre Bias (V) Collision Energy (V) Q3 Pre Bias (V) Ionization Mode Retention time (min)
THCVCOOH 315.2 271.3 25 20 19 19 Negative 1.6
315.2 163.0 25 20 29 11
THCCOOH-gluc 519.4 343.3 25 26 25 26 Negative 1.75
519.4 299.3 25 26 34 21
d3-THCCOOH-gluc 522.4 346.2 25 26 25 25 Negative 1.74
522.4 302.2 25 26 35 22
THC-gluc 489.1 313.3 25 24 30 22 Negative 1.79
489.1 112.8 25 24 21 24
THCCOOH 343.4 299.3 25 17 21 22 Negative 2.24
343.4 245.3 25 17 28 18
d9-THCCOOH 352.1 308.4 25 17 21 22 Negative 2.23
352.1 254.2 25 17 30 30
THCAA 357.1 313.3 25 17 25 22 Negative 2.76
357.1 245.1 25 17 31 17
11-OH-THC 329.4 172.9 95 16 20 23 Negative 3.84
329.4 172.9 95 16 29 30
d3-11-OH-THC 332.1 314.1 95 16 21 23 Negative 3.82
332.1 173.2 95 16 28 12
THCV 287.0 165.2 45 −20 −21 −19 Positive 5.24
287.0 231.2 45 −20 −20 −15
CBD 313.3 245.0 45 20 21 26 Negative 5.74
313.3 107.0 45 20 38 23
d3-CBD 316.1 248.1 45 21 22 27 Negative 5.73
316.1 106.9 45 21 31 22
CBG 317.2 193.1 45 −23 −17 −19 Positive 5.9
317.2 123.0 45 −23 −30 −19
CBN 309.4 279.0 45 15 30 29 Negative 6.92
309.4 222.2 45 15 46 11
d3-CBN 316.1 248.1 45 15 31 20 Negative 6.89
316.1 106.9 45 15 44 24
THC 315.2 193.1 45 −22 −23 −19 Positive 7.6
315.2 123.0 45 −22 −34 −21
d3-THC 318.2 196.2 45 −22 −23 −12 Positive 7.57
318.2 123.0 45 −22 −35 −22