. Author manuscript; available in PMC: 2017 Oct 1.

Published in final edited form as: J Chem Neuroanat. 2016 Mar 2;76(Pt B):122–132. doi: 10.1016/j.jchemneu.2016.02.004

Table 1. Stereological Parameters.

This table reflects the conditions under which neurons in the VTA and amygdala, and astrocytes of the DG were counted. SSF, section sampling fraction, is the number of sections analyzed divided by the total number of sections through the region. ASF, area sampling fraction, is the area sampled divided by the total area of the region. ΣQ-, the sum of markers counted, the total number of neurons or astrocytes counted within the regions of interest. Vref, reference volume, is the total volume measured for the region of interest. CE, the coefficient of error, a second-order stereological parameter for total observed variation, should be maintained at <0.1

	SSF	ASF	ΣQ-	Vref (mm³)	CE
CTL VTA	0.11	0.08	504	1.950	0.05
MD VTA	0.11	0.08	388	2.034	0.06
CTL Amygdala	0.11	0.01	416	6.673	0.05
MD Amygdala	0.11	0.01	536	7.101	0.04
CTL DG	0.11	0.02	341	8.836	0.04
MD DG	0.11	0.02	295	8.035	0.04