Figure 2. anti-IHF_Ec antibodies diminished pre-formed Aa biofilms in vitro.

Aa biofilms were pre-formed in vitro for 24 hours in mTSB and treated with one of the following: (A) medium only, (B) naïve serum, or (C) anti-IHF_Ec. In addition to direct treatment, similar treatments were performed with (D) PLGA microspheres that contained either (E) naïve serum (F) or anti-IHF_Ec. Representative images of Aa biofilms are shown. COMSTAT analysis was employed to calculate maximum height (G), average thickness (H) and biomass (I). (J) CFU/ml in biofilm and planktonic state was enumerated. All in vitro biofilm assays were repeated a minimum of three times on separate days, and all individual biofilm assays were done in duplicates on each assay day. Data are presented as mean values ± standard deviation. A p-value ≤ 0.05 was considered significant. p-value of 0.01–0.05 is represented by *, p-value less than 0.01 is represented by **, p-value less than 0.001 is *** and p-value less than 0.0001 is represented by ****.