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Preparation of ARP-DNA standards
| Tubes | ARP-DNA standard (µl) | Reduced DNA standard (µl) | TE buffer (µl) | Total volume (µl) | DNA concentration (µg/ml) | AP sites per 100,000 bp |
|---|---|---|---|---|---|---|
| 1 | 20 | 0 | 100 | 120 | 1 | 40 |
| 2 | 16 | 4 | 100 | 120 | 1 | 32 |
| 3 | 12 | 8 | 100 | 120 | 1 | 24 |
| 4 | 8 | 12 | 100 | 120 | 1 | 16 |
| 5 | 4 | 16 | 100 | 120 | 1 | 8 |
| 6 | 2 | 18 | 100 | 120 | 1 | 4 |
| 7 | 1 | 19 | 100 | 120 | 1 | 2 |
| 8 | 0 | 20 | 100 | 120 | 1 | 0 |
