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Effects of naltrexone (NAL), NKB, and kisspeptin (KP) on serum LH. Five healthy male participants attended for eight separate study visits at least a week apart and received vehicle, NAL, NKB, and KP alone, and in combination in random order. One-hour baseline blood sampling was conducted prior to starting the treatment. Ten-minute blood sampling was continued from the start of the treatment for 8 hours to measure gonadotrophins.
