Table 1.

Primer and probe sequences used to identify Rickettsia africae

Oligonucleotide name	Target gene	Oligonucleotide sequence (5’ → 3’)	Reference
Real time PCR
RickF	gltA gene	GGT ATA CCG TCG CAA ATG TTC AC	[7]
RickR	gltA gene	GGG TCT TCG TGCATT TCT TTC C	[7]
RickTM	gltA gene	TGT GCC ATC CAG CCT ACG GTT CTT Ga	[7]
Primers used for sequencing
Rick-F1	16S rRNA gene	GAA CGC TAT CGG TAT GCT TAA CAC A	[8]
Rick-R2	16S rRNA gene	CAT CAC TCA CTC GGT ATT GCT GGA	[8]
RCK/23-5-F	23S-5S IGS	GAT AGG TCR GRT GTG GAA GCA C	[9]
RCK/23-5-R	23S-5S IGS	TCG GGA YGG GAT CGT GTG TTT C	[9]
CS-78	gltA gene	GCA AGT ATC GGT GAG GAT GTA AT	[10]
CS-323	gltA gene	GCT TCC TTA AAA TTC AAT AAA TCA GGA T	[10]
M59	ompB (I)	CCGCAGGGTTGGTAACTGC	[11]
120-807 R	ompB (I)	CCTTTTAGATTACCGCCTAA	[11]
120-607 F	ompB (II)	AATATCGGTGACGGTCAAGG	[11]
120-1497	ompB (II)	CCTATATCGCCGGTAATT	[11]
120-1378	ompB (III)	TAAACTTGCTGACGGTACAG	[11]
120-2399	ompB (III)	CTTGTTTGTTTAATGTTACGGT	[11]
120-2113	ompB (IV)	CGATGCTAACGTAGGTTCTT	[11]
120-2988	ompB (IV)	CCGGCTATACCGCCTGTAGT	[11]
120-2788	ompB (V)	AAACAATAATCAAGGTACTGT	[11]
120-3599	ompB (V)	TACTTCCGGTTACAGCAAAGT	[11]
120-3462	ompB (VI)	CCACAGGAACTACAACCATT	[11]
120-4346	ompB (VI)	CGAAGAAGTAACGCTGACTT	[11]

^aReal time PCR probe was labeled with 5’FAM and 3’TAMRA