Table 1.
Oocyte and Embryo Characteristics of IVF and ICSI Attempts of Three Affected Individuals
| Individual | IVF and ICSI Attempts | Protocol | Total Oocytes | MII Oocytes | Fertilized Oocytes | Normal Cleavage Embryos | Viable Embryos on Day 3 | Outcomes |
|---|---|---|---|---|---|---|---|---|
| IV-5 in family 1 | first IVF | long | 9 | 7 | 6 | 6 | 2 | the two viable embryos (a grade IV 5-cell embryo and a grade III 6-cell embryo) were transferred into the uterus but failed to establish pregnancy; the remaining four embryos were arrested at the 2- to 4-cell stage on day 3 |
| second ICSI | short | 8 | 6 | 5 | 4 | 0 | all embryos were arrested at the 2- to 4-cell stage on day 3 | |
| third ICSI | prolonged | 6 | 4 | 3 | 2 | 1 | the only viable embryo proceeded to be cultivated but failed to form a blastocyst on day 5; the other embryo was arrested at the 2-cell stage on day 3 | |
| fourth ICSI | mild stimulation | 8 | 7 | 5 | 4 | 0 | all embryos were arrested at the 2- to 3-cell stage on day 3 | |
| fifth ICSI | mild stimulation | 6 | 4 | 1 | 1 | 0 | the embryo was arrested at the 3-cell stage on day 3 | |
| sixth ICSI | mild stimulation | 5 | 5 | 2 | 2 | 0 | all embryos were arrested at the 2- to 4-cell stage on day 3 | |
| II-1 in family 2 | first IVF | short | 6 | 6 | 3 | 3 | 1 | the only viable (6-cell) embryo on day 3 was further cultured but failed to form a blastocyst on day 5; the other embryos were arrested at the 2- and 5-cell stages |
| second ICSI | mild stimulation | 7 | 7 | 7 | 1 | 0 | the embryo was arrested at the 2-cell stage on day 3 | |
| third ICSI | mild stimulation | 12 | 8 | 8 | 6 | 0 | all embryos were arrested at the 3- to 4-cell stage on day 3 | |
| II-1 in family 3 | first ICSI | mild stimulation | 5 | 4 | 4 | 3 | 2 | one embryo was arrested at the 2-cell stage; two grade III 6-cell embryos were transferred into the uterus but failed to establish pregnancy |
| second ICSI | short | 7 | 5 | 5 | 3 | 0 | three embryos were arrested at the 4-cell stage on day 3 | |
| third ICSI | mild stimulation | 12 | 10 | 9 | 5 | 1 | four embryos were arrested at the 2- to 4-cell stage on day 3; one grade II 5-cell embryo was transferred into the uterus but failed to establish pregnancy | |
| fourth ICSI | mild stimulation | 13 | 11 | 11 | 9 | 1 | eight embryos were arrested at the 2- to 5-cell stage, whereas one viable embryo at the 7-cell stage was transferred into the uterus but failed to establish pregnancy | |
| fifth ICSI | mild stimulation | 7 | 2 | 2 | 2 | 0 | one embryo was arrested at the 2-cell stage on day 3, and another embryo was arrested at the 5-cell stage on day 3 |