Table 4.
Minimum inhibitory (MIC) and bactericidal concentration (MBC) of the methanolic extract (mg/mL) of Calibrachoa cultivars contents following SWE foliar (Fol) and Drench (Dre) treatments with 5 or 7 mL/plant. Values are expressed as mean of triplicate determinations ± sd
| SWE treatments | Bacillus cereus | Staphylococcus aureus | Micrococcus flavus | Enterobacter cloacae | |
|---|---|---|---|---|---|
| CHSD | MIC | ||||
| Streptomycin | 0.08 ± 0.004b | 0.20 ± 0.01a | 0.11 ± 0.01c | 0.06 ± 0.001e | |
| Ampicillin | 0.08 ± 0.003b | 0.11 ± 0.01e | 0.10 ± 0.02 cd | 0.15 ± 0.03ab | |
| CONTROL | 0.10 ± 0.001a | 0.18 ± 0.002b | 0.18 ± 0.001a | 0.17 ± 0.002a | |
| (5 ml) Fol | 0.09 ± 0.003ab | 0.17 ± 0.007bc | 0.17 ± 0.002ab | 0.16 ± 0.001a | |
| (7 ml)Fol | 0.08 ± 0.002b | 0.16 ± 0.005c | 0.15 ± 0.005b | 0.14 ± 0.001b | |
| (5 ml)Dre | 0.07 ± 0.001bc | 0.14 ± 0.005d | 0.12 ± 0.004c | 0.10 ± 0.004c | |
| (7 ml)Dre | 0.065 ± 0.003c | 0.1 ± 0.02e | 0.08 ± 0.002d | 0.08 ± 0.002d | |
| MBC | |||||
| Streptomycin | 0.11 ± 0.002de | 0.40 ± 0.003a | 0.21 ± 0.01c | 0.10 ± 0.03d | |
| Ampicillin | 0.19 ± 0.003a | 0.17 ± 0.002 g | 0.20 ± 0.01c | 0.21 ± 0.002c | |
| CONTROL | 0.17 ± 0.005b | 0.30 ± 0.001b | 0.30 ± 0.001a | 0.32 ± 0.001a | |
| (5 ml) Fol | 0.15 ± 0.001c | 0.28 ± 0.003c | 0.28 ± 0.004ab | 0.31 ± 0.005a | |
| (7 ml)Fol | 0.14 ± 0.004c | 0.26 ± 0.004d | 0.25 ± 0.001b | 0.27 ± 0.004b | |
| (5 ml)Dre | 0.12 ± 0.005d | 0.23 ± 0.005e | 0.22 ± 0.002c | 0.22 ± 0.003c | |
| (7 ml)Dre | 0.10 ± 0.002e | 0.21 ± 0.001f | 0.16 ± 0.005d | 0.19 ± 0.001c | |
| CHSF | MIC | ||||
| Streptomycin | 0.08 ± 0.004c | 0.20 ± 0.01c | 0.11 ± 0.01e | 0.06 ± 0.001e | |
| Ampicillin | 0.08 ± 0.003c | 0.11 ± 0.01e | 0.10 ± 0.02e | 0.15 ± 0.003d | |
| CONTROL | 0.14 ± 0.001a | 0.26 ± 0.004a | 0.25 ± 0.001a | 0.28 ± 0.001a | |
| (5 ml) Fol | 0.13 ± 0.002a | 0.23 ± 0.005b | 0.22 ± 0.002b | 0.25 ± 0.005b | |
| (7 ml)Fol | 0.11 ± 0.000b | 0.19 ± 0.001c | 0.19 ± 0.001c | 0.22 ± 0.004c | |
| (5 ml)Dre | 0.10 ± 0.002b | 0.17 ± 0.001 cd | 0.18 ± 0.005 cd | 0.20 ± 0.002c | |
| (7 ml)Dre | 0.08 ± 0.005c | 0.15 ± 0.002d | 0.15 ± 0.001d | 0.16 ± 0.001d | |
| MBC | |||||
| Streptomycin | 0.11 ± 0.002f | 0.40 ± 0.003b | 0.21 ± 0.01d | 0.10 ± 0.03e | |
| Ampicillin | 0.19 ± 0.003d | 0.17 ± 0.002e | 0.20 ± 0.01d | 0.21 ± 0.002d | |
| CONTROL | 0.31 ± 0.001a | 0.45 ± 0.002a | 0.47 ± 0.002a | 0.45 ± 0.001a | |
| (5 ml) Fol | 0.27 ± 0.002b | 0.40 ± 0.001b | 0.44 ± 0.001a | 0.43 ± 0.002a | |
| (7 ml)Fol | 0.24 ± 0.001c | 0.38 ± 0.001b | 0.39 ± 0.006b | 0.37 ± 0.005b | |
| (5 ml)Dre | 0.19 ± 0.002d | 0.32 ± 0.002c | 0.33 ± 0.001c | 0.34 ± 0.001b | |
| (7 ml)Dre | 0.16 ± 0.003e | 0.29 ± 0.001d | 0.30 ± 0.003c | 0.28 ± 0.002c | |
Notes: CHSD C. h. ʻ Superbells® Dreamsicleʼ, CHSF C. h. ʻSuperbells® Frost Fireʼ, Dre Drench treatment, Fol Foliar treatment
*Means followed by different letters within a column indicate significant differences between treatments based on LSD test (P ≤ 0.05)