Table 3.
The source and components of the 2,3-BDO pathway constructed and the corresponding BDO titer 1 day post-inoculation in Z. mobilis 9C
| Strain | Plasmid | Promoter | als source | Enzymes | Acetoin (g/L) | BDO (g/L) | Ethanol (g/L) |
|---|---|---|---|---|---|---|---|
| 9C-BC9 | pEZ-BC9 | Ptet | B. licheniformis | Als, AldC, Bdh | 1.6 ± 0.4 | 10.8 ± 1.1 | 26.2 ± 0.5 |
| 9C-BC10 | pEZ-BC10 | Ptet | B. subtilis | Als, AldC, Bdh | 1.1 ± 0.5 | 10.2 ± 2.0 | 27.4 ± 1.6 |
| 9C-BC11 | pEZ-BC11 | Ptet | B. subtilis | Als, AldC, Bdh | 0.7 ± 0.1 | 13.3 ± 0.7 | 24.9 ± 0.5 |
The promoter of als gene in these constructed is driven by inducible promoter Ptet. Culture condition: 40 mL RMG8 with 200 μg/mL spectinomycin in 125-mL flask, 33 °C, 120 rpm. Four replicates each without tetracycline induction