Table 2.

Primers and probes employed in the multiplex quantitative real-time PCR

Primer	Sequence
Forward primer α-globin	5′-CGACAAGACCAACGTCAAGG-3′
Reverse primer α-globin	5′-GGTCTTGGTGGTGGGGAAG-3′
α-globin probe	5′-HEX-ACATCCTCTCCAGGGCCTCCG-BHQ-3′
Forward primer β-globin	5′-GGGCACCTTTGCCACAC-3′
Reverse primer β-globin	5′-GGTGAATTCTTTGCCAAAGTGAT-3′
β-globin probe	5′-Texas Red-ACGTTGCCCAGGAGCCTGAAG-BHQ-3′
Forward primer γ-globin	5′-TGACAAGCTGCATGTGGATC-3′
Reverse primer γ-globin	5′-TTCTTTGCCGAAATGGATTGC-3′
γ-globin probe	5′-FAM-TCACCAGCACATTTCCCAGGAGC-BHQ-3′
Forward primer RPL13A	5′-GGCAATTTCTACAGAAACAAGTTG-3′
Reverse primer RPL13A	5′-GTTTTGTGGGGCAGCATACC-3′
RPL13A probe	5′-CY5-CGCACGGTCCGCCAGAAGAT-BHQ-3′

The 5′ and 3′ chromogenic molecules are underlined in the sequence