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. 2016 Sep 2;14(1):255. doi: 10.1186/s12967-016-1016-4

Table 2.

Primers and probes employed in the multiplex quantitative real-time PCR

Primer Sequence
Forward primer α-globin 5′-CGACAAGACCAACGTCAAGG-3′
Reverse primer α-globin 5′-GGTCTTGGTGGTGGGGAAG-3′
α-globin probe 5′-HEX-ACATCCTCTCCAGGGCCTCCG-BHQ-3′
Forward primer β-globin 5′-GGGCACCTTTGCCACAC-3′
Reverse primer β-globin 5′-GGTGAATTCTTTGCCAAAGTGAT-3′
β-globin probe 5′-Texas Red-ACGTTGCCCAGGAGCCTGAAG-BHQ-3′
Forward primer γ-globin 5′-TGACAAGCTGCATGTGGATC-3′
Reverse primer γ-globin 5′-TTCTTTGCCGAAATGGATTGC-3′
γ-globin probe 5′-FAM-TCACCAGCACATTTCCCAGGAGC-BHQ-3′
Forward primer RPL13A 5′-GGCAATTTCTACAGAAACAAGTTG-3′
Reverse primer RPL13A 5′-GTTTTGTGGGGCAGCATACC-3′
RPL13A probe 5′-CY5-CGCACGGTCCGCCAGAAGAT-BHQ-3′

The 5′ and 3′ chromogenic molecules are underlined in the sequence