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. Author manuscript; available in PMC: 2016 Sep 3.
Published in final edited form as: Nat Neurosci. 2015 Nov 23;19(1):84–93. doi: 10.1038/nn.4176

Figure 7. IgSF11 has no effect on the functional properties of AMPARs.

Figure 7

(a) IgSF11 coexpression does not affect the deactivation time constant of GluA1 currents evoked by brief applications (1 ms) of glutamate (10 mM). Outside-out patches from HEK293FT cells expressing GluA1i (flip version) alone, or GluA1i + HA-IgSF11, were recorded of GluA1 currents using ultrafast perfusion. Currents were normalized for the analysis of time constants. n = 7 for GluA1i, and 5 for GluA1i + HA-IgSF11, ns, not significant. Student’s t-test.

(b) IgSF11 coexpression does not affect the desensitization time constant of GluA1 currents evoked by a longer application (1 s) of glutamate (10 mM). HEK293FT cells expressing GluA1i alone, or GluA1i + HA-IgSF11, were recorded of GluA1 currents, followed by quantitative analysis of desensitization time constants. n = 15 for GluA1i, and 8 for GluA1i + HA-IgSF11, ns, not significant, Student’s t-test.

(c) IgSF11 coexpression does not affect recovery of GluA1 currents from desensitization. HEK293FT cells expressing GluA1i alone, or GluA1i + HA-IgSF11, were recorded and superimposed of the GluA1-mediated currents for responses to brief applications of glutamate with varying time intervals, followed by quantitative analysis of recovery from desensitization (inset, examples of current traces) and time constant of recovery. n = 11 for GluA1i, and 9 for GluA1i + HA-IgSF11, ns, not significant, Student’s t-test.