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. Author manuscript; available in PMC: 2016 Sep 3.
Published in final edited form as: Mol Cell Endocrinol. 2010 Jul 23;328(1-2):93–103. doi: 10.1016/j.mce.2010.07.018

Figure 2.

Figure 2

Concentration-dependent increase in Star mRNA expression in immortalized KK1 mouse granulosa cells treated with vasoactive intestinal peptide (VIP) against the background PKA activity. A) Star mRNA expression as determined by Real Time (TaqMan) PCR normalised against the GAPDH. One-way ANOVA with p < 0.0001 (A,B,C) followed by Dunnett’s multiple comparison test was applied; all samples were compared against the untreated control. (**) indicates p < 0.01 and (***) inicates p < 0.05. B) VIP increases Star promoter activity in immortalized KK1 mouse granulosa cells. Cells were transfected with −151/−1 bp fragment of Star promoter subcloned to pGL2 vector containing Firefly luciferase as a reporter. Transfection efficiency was normalized by cotransfecting pRL-SV40 vector constitutively expressing Renilla luciferase. Cells were treated for 6 h. One-way ANOVA with p < 0.0025 and Dunnett’s multiple comparison test were applied; all samples were compared against the untreated control. Bars with (**) differ at p < 0.01.