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. Author manuscript; available in PMC: 2016 Sep 3.
Published in final edited form as: Mol Cell Endocrinol. 2010 Jul 23;328(1-2):93–103. doi: 10.1016/j.mce.2010.07.018

Figure 3.

Figure 3

Vasoactive intestinal peptide (VIP) increases Star gene expression and steroidogenesis in primary mouse granulosa cells. Mature female CD-1 strain mice were stimulated with 8 IE pregnant mare serum gonadotropin (PMSG). After isolation cells were cultivated for 24h in culture medium and subsequently stimulated for 6 h in serum free media containing either 1mM dbcAMP or 1µM VIP. A) Progesterone production in the collected media was determined by radioimmunoassay B) Star mRNA expression as determined by Real Time (TaqMan) PCR normalised against the GAPDH. One-way ANOVA with p < 0.0001 (A,B) followed by Dunnett’s multiple comparison test was applied; all samples were compared against the untreated control. (**) indicates p < 0.01.