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. Author manuscript; available in PMC: 2016 Sep 3.
Published in final edited form as: Mol Cell Endocrinol. 2010 Jul 23;328(1-2):93–103. doi: 10.1016/j.mce.2010.07.018

Figure 5.

Figure 5

Role of PKA in vasoactive intestinal peptide (VIP) – mediated STAR expression and steroidogenesis in immortalized KK1 mouse granulosa cells. KK1 cells were treated for 6 h with 1.0 µM VIP with or without 0.3 mM dbcAMP. PKA activity was blocked with H89 (25 µM). Untreated cells were used as negative control. A) Star mRNA expression as determined by Real Time (TaqMan) PCR normalised against the GAPDH. B) STAR (30kDa), P-STAR (30 kDa) and GAPDH (37 kDa) levels were examined by Western blot analysis. The average integrated optical density (IOD) for STAR, normalized with GAPDH, is shown as fold changes relative to the untreated control. C) Progesterone production in the collected media was determined by radioimmunoassay. One-way ANOVA with p < 0.0001 (A,C) followed by Dunnett’s multiple comparison test was applied; all samples were compared against the untreated control. Bars with (**) differ at p < 0.01.