Fig. 2.

Representative gating strategy for identifying NK92 and target cell conjugates. Cells were stained and data collected as described in Section 2. Conjugates were identified using IDEAS software. A) Events in focus were identified as those with high contrast values and labeled as “In Focus”. B) Events with a target cell stain within the “In Focus” group were labeled as “Target⁺”. C) Lysotracker Red⁺ and CD107a⁺ events within the “Target⁺” population were labeled as “Degranulated NK cell”. D) Events with low circulatory scores were identified as NK-target conjugates and their microscopy images were subsequently quantitatively analyzed.