Figure 3.

MiR-21 inhibitor treatment down-regulate the expression of MMP-1, MMP-3 and MMP-13. FLS cells were first treated with miR-21 NC or inhibitor, and then cells were harvested and the expression of MMP-1, MMP-3 and MMP-13 was determined by Western blot. (A) One representative result out of three is shown. (B) Gray-scale of the immune-bands were measured by Quantity One software and the relative expression of MMP-1, MMP-3 and MMP-13 to the internal control GAPDH was calculated. Data shown are mean±SD of three independent experiments. **, P<0.01