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. Author manuscript; available in PMC: 2016 Sep 4.
Published in final edited form as: Methods Mol Biol. 2011;741:301–317. doi: 10.1007/978-1-61779-117-8_20

Figure 3.

Figure 3

Hrs knockdown impedes the rΔF508 CFTR delivery into lysosomes. Postendocytic targeting of internalized CFTRs was monitored by vesicular pH (pHv) measurement on HeLa cells transiently expressing the indicated CFTR variant as described in Method 3.5. Anti-HA antibody and FITC-conjugated secondary Fab was bound for 1 h at 0°C to HeLa expressing the shHrs, as well as the wt or rΔF508 CFTR. Cells were chased for 1 h at 37C prior to FRIA. Data were obtained on indicated number of vesicles on 10-15 fields (means ± SEM). The mean pHv of the subpopulations are depicted.