Figure 3.
15N NMR titration of SipD with 5-hydroxyindole. A) Overlay of five 2D 1H-15N TROSY spectra of 15N/ILV-labeled SipD titrated with increasing molar ratios of 5-hydroxyindole, with expanded sections for selected SipD residues affected by the interaction with 5-hydroxyindole. B) Plot of chemical shift deviation (CSD) vs concentration of 5-hydroxyindole for selected residues.