. 2016 May 23;16(6):e34432. doi: 10.5812/hepatmon.34432

Table 1. Working Parameters for Western Blot Analysis^a.

Item	Primary Antibody Dilution Rate	Second Antibody Dilution Rate	Duration of Autoradiography, min
TLR3	1:100 (goat ant-human CD86 antibody)	1:1500 (mouse ant-goat IG antibody)	360
β-Actin	1:5000 (mouse ant-human β-actin antibody)	1:15000 (goat ant-mouse IG antibody)	10

^aTLR3 is a low concentration protein. The duration of autoradiography for TLR3 and β-Actin was determined by the duration of the experiment (i.e., 10, 20, 40, 60, 120, 180, 240, 300, 360, or 420 minutes), for which the optimal duration was used for both TLR3 and β-actin proteins.

Table 1. Working Parameters for Western Blot Analysisa.

Table 1. Working Parameters for Western Blot Analysis^a.