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. 2016 Jul 12;8(8):2266–2287. doi: 10.1093/gbe/evw149

Table 1.

Summarization of the Transcriptomic and Proteomic Analyses of P. mertensi Venom

Transcriptome
 Proteome
Venom Protein Family ORF Size Expression Level
 ESTs Coverage Peptides Identified
 MS/MS Coverage Spectral Count Relative Abundance
ID bp Full/ Partial reads RPKM % of venom % of all % of ORF in gel In solution % No of spectra NSAF
Kunitz type protein (KUN) 25340 133808.5 43.89 12.120
    PMERREF_KUNITZ01 258 Full 9332 49578.4 16.26 4.491 100 1 5 52.9 10 0.0320
    PMERREF_KUNITZ02 261 Full 13365 70188.5 23.02 6.358 100 1 4 45.3 9 0.0284
    PMERREF_KUNITZ03 258 Full 2643 14041.6 4.61 1.272 100 2 5 55.3 11 0.0352
Snake venom metalloproteinase (SVMP) 84619 66786.7 21.90 6.050
    PMERREF_SVMP01 1356 Full 14277 14431.6 4.73 0.013 96 11 31.3 60 0.0473
    PMERREF_SVMP02 1623 Part 5' 1147 968.7 0.32 0.001 21 3 7.8 9 0.0053
    PMERREF_SVMP03 1872 Full 3809 2789.0 0.91 0.003 68 1 26 45.5 85 0.0404
    PMERREF_SVMP04 1875 Full 3698 2703.4 0.89 0.002 51 12 26 46.3 81 0.0385
    PMERREF_SVMP05 1872 Full 10780 7893.2 2.59 0.007 100 24 44.4 89 0.0424
    PMERREF_SVMP06 1455 Part 5' 2560 2411.6 0.79 0.002 0 7 18.0 15 0.0105
    PMERREF_SVMP07 1875 Full 7726 5648.0 1.85 0.005 95 1 17 30.3 25 0.0119
    PMERREF_SVMP08 1851 Full 2210 1636.5 0.54 0.001 73 1 9 15.7 13 0.0063
    PMERREF_SVMP09 1875 Full 3356 2453.3 0.80 0.002 29 18 30.0 39 0.0186
    PMERREF_SVMP10 1848 Full 10401 7714.6 2.53 0.007 82 20 40.8 40 0.0194
    PMERREF_SVMP11 1714 Part 3' 1504 1202.8 0.39 0.001 18 15 23.3 43 0.0233
    PMERREF_SVMP12 1875 Full 13591 9935.5 3.26 0.009 98 1 22 38.0 83 0.0395
    PMERREF_SVMP13 1863 Full 2113 1554.6 0.51 0.001 29 11 23.2 19 0.0091
    PMERREF_SVMP14 1875 Full 7447 5444.0 1.79 0.005 51 26 44.9 86 0.0404
C-type lectin (CTL) 22870 66069.7 21.67 5.985
    PMERREF_CTL01 453 Full 7741 23422.7 7.68 2.122 100.0 9 7 68.7 18 0.0288
    PMERREF_CTL02 483 Full 4604 13065.5 4.29 1.183 100.0 12 68.1 20 0.0297
    PMERREF_CTL03 498 Full 2257 6212.1 2.04 0.563 100.0 9 49.1 12 0.0172
    PMERREF_CTL04 474 Full 5446 15748.4 5.17 1.426 100.0 12 11 73.9 22 0.0334
    PMERREF_CTL05 513 Full 938 2506.2 0.82 0.227 96.7 0 – 0 0
    PMERREF_CTL06 498 Full 705 1940.4 0.64 0.176 76.9 5 30.9 5 0.0072
    PMERREF_CTL07 525 Full 382 997.3 0.33 0.090 100.0 0 – 0 0
    PMERREF_CTL08 483 Full 1 2.8 0.00 0.000 43.7 0 – 0 0
    PMERREF_CTL09 528 Full 213 553.0 0.18 0.050 0.0 0 – 0 0
    PMERREF_CTL10 489 Full 232 650.3 0.21 0.059 93.3 0 – 0 0
    PMERREF_CTL11 495 Full 174 481.8 0.16 0.044 0.0 0 – 0 0
    PMERREF_CTL12 495 Full 113 312.9 0.10 0.028 0.0 0 – 0 0
    PMERREF_CTL13 498 Full 64 176.1 0.06 0.016 66.9 0 – 0 0
Acid lipase (svLIPA)
    PMERREF_LIPA01 1200 Full 6931 8582.0 2.81 0.777 100.0 2 18 70.9 42 0.0251
L-amino acid oxidase (LAAO)
    PMERREF_LAAO01 1460 Full 7565 7068.3 2.32 0.640 62.6 54 33 63.4 73 0.0324
Three-finger toxin (3FTx) 1013 5370.9 1.76 48.650
    PMERREF_3FTX01 361 Full 902 4737.0 1.55 0.429 100 1 12.8 1 0.0032
    PMERREF_3FTX02 241 Full 111 633.9 0.21 0.057 0 0 – 0 0
Waprin (WAP)
    PMERREF_WAP01 240 Full 667 3809.4 1.25 0.345 100 0 - 0 0
Defensin 563 3976.1 1.30 36.015
    PMERREF_DEF01 195 Full 515 3638.7 1.19 0.330 100 4 46.9 3 0.0142
    PMERREF_DEF02 195 Full 48 337.4 0.11 0.031 0 0 – 0 0
Gamma PLA2 inhibitor (gPLA2i)
    PMERREF_GPLA2I01 384 Full 597 2131.0 0.70 0.193 0 1 12.6 0 –
Phospholipase B (PLB)
    PMERREF_PLB01 1662 Full 135 1478.72 0.48 0.134 0 18 39.8 19 0.0074
Ku-wap-fusin (KUWAPF)
 PMERREF_KUWAPF01 387 Full 317 1122.8 0.37 0.102 0 0 – 0 0
Cysteine-rich secretory protein (CRISP)
    PMERREF_CRISP01 780 Full 527 1003.3 0.33 0.091 0 8 42.3 11 0.0104
Ohanin-like (OHANIN)
    PMERREF_OHAN01 642 Full 380 811.3 0.27 0.073 69.3 5 32.4 6 0.0065
Serine proteinase (SVSP)
    PMERREF_SVSP01 771 Full 369 656.0 0.22 0.059 0 9 43.8 11 0.0099
Cystatin 191 618.4 0.20 5.602
    PMERREF_CYST01 426 Full 158 508.4 0.17 0.046 0 3 30.5 4 0.0069
    PMERREF_CYST02 411 Full 33 110.1 0.04 0.010 0 0 – 0 0
Matrix metalloproteinase (svMMP)
    PMERREF_MMP01 1986 Part 632 436.2 0.14 0.040 0 24 34 61.3 61 0.0192
C-type natriuretic peptide (CNP)
    PMERREF_CNP01 861 Full 277 441.0 0.14 0.040 0 1 – 1 –
Cobra venom Factor-like (CVF)
    PMERREF_CVF01 1242 Part 5' 195 215.2 0.07 0.019 0 6 16.7 6 0.0030
Beta PLA2 inhibitor (bPLA2i)
    PMERREF_BPLA2I01 918 Part 5' 135 201.6 0.07 0.018 0 2 6.9 2 0.0015
5' nucleotidase (5NUCLEO)
    PMERREF_5NUCLE01 1575 Part 5' 216 188.0 0.06 0.017 59.4 1 18 42.4 21 0.0087
Vascular endothelial groth factor (svVEGF)
    PMERREF_VEGF01 579 Full 50 118.4 0.04 0.011 0 1 15.1 1 0.0013

Note.—The assembled transcripts coding for known snake venom proteins are listed according to their expression levels (RPKM), showing the corresponding sequence features and proteomic identification in the venom.