Fig. 2.—

Conserved sites of protein coding genes. Single genes were aligned, masked using four different approaches, and finally only sites selected as phylogenetically informative by at least three softwares were kept. Whenever possible in terms of sample size, the same procedure was carried out for reduced datasets, corresponding to single subclasses (3 datasets) and single families (5 families). Each chart shows the proportion of datasets keeping single sites: this is 0 (discarded site) or 100 (kept site) at the class level, and ranges from 0 to 100 at the subclass and family level, because more datasets are available.