Figure 6.

KPT-9274 attenuates xenograft growth in vivo with minimal toxicity and shows the expected on-target effects.

A. Nude mice (n=8 per condition) were xenografted subcutaneously with 786-O cells and gavaged with KPT-9274 (100mg/kg or 200 mg/kg twice a day). Tumors were measured with calipers weekly at the times indicated and the mean tumor volumes ± SEM was calculated as described in Materials and Methods.

**p<0.05 indicates significant decrease in tumor volume compared to vehicle only group.

B. Mouse weights were determined at the time points indicated.

C. Tumors were harvested at sacrifice, pooled, and immunoblotted with the target antibodies indicated as well as β-actin as loading control.