Fig. 3.
BDS increases the number of CD11b-positive cells in the blood of PND14, but not PND28 mice. (A) Representative bivariate dot plots of SSC-A/CD11b (CD11b-positive cells) and SSC-A/Ly6C (granulocytes and monocytes) labeling of blood cells obtained on PND14 and PND28. Gating for CD11b/SSChi/Ly6Cint (granulocytes) and CD11b/SSClow/Ly6Chi (monocytes) is shown on the SSC-A/Ly6c dot plots. BDS increased the total number of CD11b-positive cells (B) and CD11b/SSChi/Ly6Cint-granulocytes (C) at PND14, but not PND28. There was no effect of age or BDS on the percentage of CD11b/SSClow/Ly6Chi monocytes (D). N = 7–10 mice, from 7 to 8 different litters, per group. Error bars represent mean ± SEM. *p < 0.05, **p < 0.01.