Skip to main content
. Author manuscript; available in PMC: 2017 Sep 15.
Published in final edited form as: J Immunol. 2016 Aug 17;197(6):2400–2408. doi: 10.4049/jimmunol.1600138

Figure 1. IL-17 activates PCs, but not ECs, to produce pro-inflammatory molecules.

Figure 1

(A) HUVEC and PC production of IL-6 and IL-8 transcripts after 6h or 24 h of IL-17 (20ng/ml) treatment assessed by qRT-PCR and normalized to GAPDH. Fold increase of the IL-17-stimulated conditions was calculated based on unstimulated controls of the respective cell type. Unstimulated control ECs and PCs express comparable levels of IL-6 and IL-8 transcripts. The threshold cycle numbers (Ct) for all conditions were below 30 cycles. (B) HUVEC and PC production of IL-6 and IL-8 proteins assessed by ELISA after 24h of IL-17 (20ng/ml) treatment. [n=3-6, t-test, SEM] *p<0.05, **p<0.01, ***p<0.001.