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. Author manuscript; available in PMC: 2017 Sep 15.
Published in final edited form as: J Immunol. 2016 Aug 15;197(6):2187–2194. doi: 10.4049/jimmunol.1600793

Figure 4. Axl-mediated Akt and Bcl-xl activation in the kidney of nephritic mice.

Figure 4

Glomerulonephritis was induced as described. Kidney samples were homogenized and prepared in RIPA buffer. Immunoblotting analysis compared expression and activity of Axl signaling pathway molecules (Akt) between WT, Axl-KO, Mer-KO, and A/M-KO mice at day 3 (A) and day 14 (B). Bcl2, Bcl-6, and Bcl-xl expression were also analyzed at day 3 (C) and day 14 (D) after injection. Densitometry analysis to quantify the ratio of phosphorylated Akt to total Akt and Bcl proteins to β-actin, respectively, is shown. Representative Western images are shown. Experiments were independently repeated at least 2 times. Values are expressed as median with interquartile range. Statistical test is the Mann-Whitney test. **p<0.01, *p<0.05.