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. Author manuscript; available in PMC: 2017 Sep 15.
Published in final edited form as: J Immunol. 2016 Aug 8;197(6):2509–2521. doi: 10.4049/jimmunol.1502659

Figure 4. Nur77GFPhi CD8+ tumor infiltrating T cells make IFNγ.

Figure 4

Nur77GFP mice were inoculated with MCA205 tumor cells on the hind flanks. ~12 days after tumor inoculation in situ IFNγ was assessed 6 hours after animals were injected with Brefeldin A or PBS alone. A) IFNγ production by T cells in tumor draining lymph nodes and B) tumor. The histogram overlays are representative of GFP expression from IFNγ- (shaded grey) and IFNγ+ (black line) populations of CD8 T cells. C) Percentage of IFNγ producing CD8 T cells in GFPhi vs GFPlow T cell subsets from tumor and dLN. D) IFNγ MFI in TCRβ+CD8+Nur77GFP subsets from the tumor. Data are representative of 3 experiments with n=3 animals per treatment group. Unpaired two-tailed Student’s t test was used for statistical analysis of two groups and graphed as mean with SEM. For more than two groups, a one-way ANOVA multiple comparisons analysis was used and *p≤0.0001.