Table I.
Antibodies used for flow cytometry
| Antigen | Color | Clone | Host Species | Isotype | Supplier |
|---|---|---|---|---|---|
| CD2 | Qdot605 | S5.5 | Mouse | IgG2a | Invitrogen |
| CD3 | BV605 | SP34-2 | Mouse | IgG1,λ | BD Bioscience |
| CD14 | Qdot605/Qdot800 | Tu14 | Mouse | IgG2a | Invitrogen |
| CD19 | PE-Cy5 | J3-119 | Mouse | IgG1 | Beckman Coulter |
| CD20 | eF650NC | 2H7 | Mouse | IgG2b,κ | eBioscience |
| CD21 | PE-Cy7 | B-ly4 | Mouse | IgG1,κ | BD Bioscience |
| CD27 | PerCP-eF710 | O323 | Mouse | IgG1,κ | eBioscience |
| CD138 | PE | DL-101 | Mouse | IgG1,κ | Biolegend |
| Biotinylated SIVmac251ENV | NA | NA | NA | NA | NA |
| IgD | Texas Red | Polyclonal | Goat | NA | Southern Biotech |
| IgG | APC-Cy7 | G18-145 | Mouse | IgG1,κ | BD Bioscience |
| Ki-67 | A×700 | B56 | Mouse | IgG1,κ | BD Bioscience |
| IRF-4 | eFluor660/FITC | 3E4 | Rat | IgG1,κ | eBioscience |
| HLA-DR | Qdot800 | Tu36 | Mouse | IgG2b | Invitrogen |
| CD25 | PE-Cy7 | BC96 | Mouse | IgG1,κ | eBioscience |
| CD3 | PE | SP34-2 | Mouse | IgG1, λ | BD Bioscience |
| CD4 | APC | L200 | Mouse | IgG1,κ | BD Bioscience |
| Viability Dye | Aqua | NA | NA | NA | Invitrogen |