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. Author manuscript; available in PMC: 2017 Sep 15.
Published in final edited form as: J Immunol. 2016 Aug 15;197(6):2382–2389. doi: 10.4049/jimmunol.1600994

Figure 4. Charon is required for Rel68 protein binding in salivary gland polytene chromosomes.

Figure 4

A. Charon and Relish proteins are co-localized in chromatin: Drosophila larvae expressing Charon-GFP were cultured for 60hrs with E.coli bacteria; salivary glands were dissected from third instar larvae, squashed and stained with monoclonal anti-GFP (Red) and polyclonal anti-Relish (green) antibodies; DNA was detected using propidium iodide (blue). B–C. Charon is required for Rel68 protein binding in chromatin: wild type (WT) or Charon knockdown (siRNACG5118) larvae were treated as described above; the localization of Relish in polytene chromosomes was detected using the polyclonal anti-Relish (green) antibodies; DNA was detected using propidium iodide (red).