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. Author manuscript; available in PMC: 2017 Sep 15.
Published in final edited form as: J Immunol. 2016 Aug 12;197(6):2079–2089. doi: 10.4049/jimmunol.1501805

Figure 7. Reduced numbers of CD8 TRM cells in the absence of ADAP blunts the response to local antigen challenge, but not recruitment of circulating memory CD8 T cells.

Figure 7

Naïve WT (CD45.2) or ADAP−/− (CD45.2) OT-I T cells were single transferred into CD45.1 hosts, and challenged with VSV-OVA. (A–D) On day 30+ post infection, animals were t.c. challenged with SIINFEKL peptide. At 12 hours post challenge, FRTs were harvested and stained for cell surface markers and intracellular cytokines. (A) Representative IFN-γ staining from WT or ADAP−/− OT-I T cells. (B) Percentage of IFN-γ+ WT (black bars) or ADAP−/− (white bars) OT-I T cells. (C) Representative Granzyme B staining from WT or ADAP−/− OT-I T cells. (D) Fold change in gMFI of Granzyme B+ WT or ADAP−/− OT-I T cells over unstimulated. (E) On day 30+ post infection animals received P14 memory cells, followed by t.c. challenge with SIINFEKL peptide. At 48 hours post challenge FRTs were harvested and stained for cell surface markers. (E) Number of circulating memory P14 cells recruited into the FRT. The results (B, D and E) are representative of 2 independent experiments, with 3 mice per group (± SEM). *, p < 0.05.