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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Jul 11;1860(11 Pt A):2363–2376. doi: 10.1016/j.bbagen.2016.07.004

Fig. 4. Baseline glutathione levels and antioxidants defenses.

Fig. 4

(A) GSH and GSSG levels of TIB-73 and TIB-75 cell pellets were determined using an enzymatic recycling method. TIB-75 cells had a significantly lower GSH:GSSG ratio compared to TIB-73 cells (p-value < 0.0001, n = 5). (B) Antioxidant protein levels from TIB-73 and TIB-75 cell lysates were determined by Western Blot and normalized to β-actin protein. SOD-1 protein expression was not significantly different (p-value = 0.50, n = 8), but TIB-75 cells had significantly lower GPx-4 (p-value = 0.004, n = 10) and catalase (p-value =0.009, n = 5) protein expression compared to TIB-73 cells. * = p-value ≤ 0.05, ** = p-value ≤ 0.01, *** = p-value ≤ 0.001, **** = p-value ≤ 0.0001. The error bars represent the standard error of the mean.