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. Author manuscript; available in PMC: 2017 Sep 15.
Published in final edited form as: J Immunol. 2016 Aug 10;197(6):2338–2352. doi: 10.4049/jimmunol.1600574

Figure 9. Survival of CD8+ T cells following viral dissemination to the MLN.

Figure 9

(A) HSV-1 titer in the MLN at day 5 pi (n = 7–8 mice per group; 3 independent experiments; ND, not detected). Total amounts of cytokines associated with antiviral defense including IFNα2,4 (B) and IFNγ (C); the immunomodulatory growth factor VEGF A (D); T cell differentiation or survival including IL-2 (E), IL-6 (F), IL-7 (G), IL-12p70 (H), and IL-15 (I) were evaluated at day 5 pi (n = 9 mice per group; 3 independent experiments). Stastical differences for panels A – I were determined by one-way ANOVA with Student-Newman-Keuls multiple comparisons tests; * and ^ reflect differences from WT and STING−/−, respectively. (J) Viability of CD8+ T cells recovered from MLN on day 5 pi assessed by propidium iodide and Annexin V staining (n = 5–9 mice per group; 2–3 independent experiments); parameters for determination of cell viability are shown in inset dot plot; differences were determined by two- way ANOVA with Bonferroni post tests; significance thresholds are as follows: p < 0.05 = *, p < 0.01 = **, p < 0.001 = ***. Bars represent mean ± SEM.