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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Transplantation. 2017 Jan;101(1):131–140. doi: 10.1097/TP.0000000000001076

Figure 1.

Figure 1

Upper panel with four scatterplots shows increased risk of rejection, because CD154+TcM induced by stimulation with donor PBL exceed those produced after stimulation with HLA-non-identical PBL in the reference reaction. Lower panel with four scatterplots shows decreased risk of rejection, because donor-induced CD154+TcM are exceeded by those in the reference reaction. The antibody to CD154 is labeled with the fluorochrome, phycoerythrin. T-cytotoxic memory cells which express CD154 (green dots) are separated from those that do not express CD154 (magenta dots) by implementing the gating strategy described in Supplementary Figure 1 in negative control reaction condition. SSC = side scatter.