Table 1A.

Subject and sample characteristics. Organ type L=liver, LSB=liver-intestine, SB=intestine, LK=liver-kidney. Sample type IR0=pre-transplant sample. IR1=sample obtained on post-transplant days 1-60. IRx=sample obtained on post-days 200-onward. A negative prefix for time between sample and transplant denotes a pre-transplant sample obtained before transplantation. The time between sample and biopsy/event is preceded by a negative symbol or is zero because all samples were obtained before or on the day of the biopsy/event.

	Training set( August 10, 2006 to June 9. 2010)		Validation set( March 19, 2009 to August 16, 2012)
	All	Analyzable	All	Analyzable	p- valuetest vs validation(All subjects)
Parameters
Total subjects	127	120	87	72
Subject Age (Years) (mean +/-SD, range)	8.0±6.3,0.2 to 20.9	8.0±6.3,0.2 to 20.9	8.6±6.7,0.4 to 20.2	8.7±6.6,0.4 to 20.1	NS (0.502)
Gender (M:F)	81:46	76:44	48:39	41:31	NS (0.255)
Race (Caucasian: non-Caucasian)	92:35	86:34	68:19	55:17	NS (0.423)
Organ (L: LSB: SB: LK: LL)	83:24:17:3:0	79:22:16:3:0	71:5:10:0:1	61:2:8:0:1	0.007
Induction (None: thymo: campath)	41:73:13	38:69:13	38:46:3	32:37:3	NS (0.080)
FKWB ( ng / ml )	9.5 ± 6.3	9.3 ± 6.1	7.9 ± 5.1	7.5 ± 4.6	NS (0.095)
Actual donor: surrogate donor	24 :103	24 : 96	6 : 81	5 : 67	0.015
Total samples	158	147	122	97
IR0 samples	50	49	43	33
IR1 samples	54	48	39	30
IRx samples	54	50	40	34
Time between Transplantation and pre-transplant sample (days) IR0 / (range)	-0.4 ±2(-14 to 0)	-0.4 ±2(-14 to 0)	-4.1 ±15.6(-85 to 0)	-5.2 ±17.7(-85 to 0)
Time between Transplantation and post-transplant samples (days) IR1+IRx	1183 ± 1786(7 to 6226)	1202 ± 1825(7 to 6226)	938± 1435(6 to 5360)	989 ± 1474(6 to 5360)	NS (0.336)
Time between sample and biopsy/event (days) / (range)	14.4±14.5( 0 to 59)	14.8 ± 14.4(0 to 59)	15.2±17.2( 0 to 59)	13.8±17.0(0 to 59)	NS (0.769)
Exclusions
Failure to generate signal	11		16
Technical failure (inadequate cell counts)	0		9