Table 3. Effect of multiple antibodies on %CD8+cells labeled with anti-CD8-APCH7.
| Normal control 1 | Normal control 2 | Normal control 3 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Lot 1 | Lot 2 | Lot 1 | Lot 2 | Lot 1 | Lot 2 | |||||||
| Tube 1 | CD8-APCH7 | 19.6 | 18.7 | 30.8 | 30.1 | 28.7 | 29.2 | |||||
| Tube 2 | CD8-APCH7 | CD8-PECy7 | 18 | 17.6 | 31 | 29.2 | 39.4 | 32.4 | ||||
| Tube 3 | CD8-APCH7 | CD3-FITC | 18.6 | 19.3 | 32.4 | 30.9 | 30.7 | 31.4 | ||||
| Tube 4 | CD8-APCH7 | CD45RO-APC | 18.2 | 19.6 | 32.7 | 33.4 | 29.6 | 32.7 | ||||
| Tube 5 | CD8-APCH7 | 7-AAD | 18.1 | 19.2 | 31.2 | 31.3 | 30.3 | 31.6 | ||||
| Tube 6 | CD8-APCH7 | CD154 | 16.1 | 16.6 | 25.6 | 25.4 | 28.5 | 27 | ||||
| Tube 7 | CD8-APCH7 | CD3-FITC | CD45RO-APC | 18.4 | 19.3 | 32.8 | 32.9 | 28.3 | 28.5 | |||
| Tube 8 | CD8-APCH7 | CD8-PECy7 | CD3-FITC | CD45RO-APC | 18.4 | 18.4 | 27.4 | 31.4 | 34.6 | 35.8 | ||
| Tube 9 | CD8-APCH7 | CD8-PECy7 | CD3-FITC | CD45RO-APC | 7-AAD | 18.6 | 18 | 27.4 | 29.8 | 35.4 | 32.6 | |
| Tube 10 | CD8-APCH7 | CD8-PECy7 | CD3-FITC | CD45RO-APC | 7-AAD | CD154 | 19.8 | 17.8 | 27.2 | 30.8 | 35 | 33.4 |
| Mean | 18.38 | 18.45 | 29.85 | 30.52 | 32.05 | 31.46 | ||||||
| SD | 1.00 | 0.95 | 2.68 | 2.22 | 3.79 | 2.58 | ||||||
| %CV | 5.5 | 5.2 | 9.0 | 7.3 | 11.8 | 8.2 | ||||||