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. 2016 Sep 1;99(3):770–776. doi: 10.1016/j.ajhg.2016.07.009

Figure 5.

Figure 5

CEP78 in Human Cells and Its Interaction with FAM161A

(A) Immunostaining of CEP78 (red dots) and of cone arrestin (green) in a section of human retina. Margins of a cone photoreceptor are highlighted by a dotted line. The scale bar represents 10 μm.

(B). Co-immunoprecipitation of endogenous CEP78 and FLAG-tagged FAM161A in HEK293T cells. FLAG-SOX4 is a negative control. Abbreviations are as follows: IP:x, protein targeted by the antibody used in immunoprecipitation; and WB:x, protein or peptide targeted by the antibody used in immunoblots. The single and double asterisks indicate the expected molecular sizes for CEP78 (78 kDa) and the FLAG-FAM161A construct (90 kDa), respectively.

(C) Staining of CEP78 (red) and acetylated tubulin (green) in fibroblasts from a control individual, KN10, and 2702r34. CEP78 localizes at the centrioles and at the base of the induced primary cilium. The scale bars represent 5 and 1 μm for regular and magnification panels, respectively.