Figure 5.
PLS3 Interacts with CORO1C and TMOD3 in HEK293T Cells, but only CORO1C Directly Interacts with PLS3
(A and B) Immunoblots of co-IP experiments show the interaction of PLS3 with CORO1C (A) and TMOD3 (B).
(C) Pull-down experiments with His-TMOD3 and GST-PLS3. Immunoblots probed with GST and TMOD3 antibodies show that PLS3 does not interact directly with TMOD3.
(D) Pull-down experiments with EGFP-CORO1C and GST-PLS3 show direct interaction of PLS3 with CORO1C.
(E) PLS3-CORO1C interaction is disrupted in the presence of 1 mM Ca+2.
(F) Pull-down assay of purified protein domains of CORO1C with GST-PLS3 shows that the interaction of PLS3 and CORO1C is mediated through the N-terminal part of CORO1C.