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. 2016 Jul 26;18(9):726–734. doi: 10.1016/j.hpb.2016.06.015

Figure 4.

Figure 4

Analysis of cell death and tissue architecture in the liver following high frequency irreversible electroporation (H-FIRE). Apoptotic and necrotic tissue area calculated following TTC staining of tissue. *p < 0.05 200- and 300-pulses versus 100-pulses, n = 5. b) Representative H&E and immunohistochemistry staining using an anti-caspase-3 antibody illustrating region between H-FIRE (HF) and normal liver (NL) following 200-pulse H-FIRE delivery (2250 V, 2-5-2 pulse configuration, 1.5 cm spacing) (×40 and ×100). Caspase 3 activity/apoptosis stains brown. c) Blind scoring (score scale 0–4) of caspase-3 staining in liver tissue following 100-, 200- and 300-pulse delivery (2250 V, 2-5-2 pulse configuration, 1.5 cm spacing). *p < 0.05 versus normal tissue (no H-FIRE), #p < 0.05 200- and 300-pulses versus 100-pulses, $p < 0.05 200- versus 300-pulses. d) Representative image (×40 and ×100) highlighting immune cell infiltration in ablated regions following 300-pulse H-FIRE delivery (2250 V, 2-5-2 pulse configuration, 1.5 cm spacing)