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. 2016 Sep 5;7:1374. doi: 10.3389/fmicb.2016.01374

Table 3.

Kinetic parameters of available SHV-type extended-spectrum β-lactamases.

Enzyme Parameter PEN AMP AMX TIC PIP CER CEF CAZ CTX FEP ATM CLA SUL TZB
SHV-1** Kcat 455 900 60 570 170 10 NH NH >100 NH
Km 20 90 22 60 110 26 ND ND >3000 ND
Kcat/Km 23,000 10,000 2700 10,000 1500 400 ND ND >35 ND
Vmax/Km 100 4 1
Ki 0.19 1.70 0.057
IC50 0.057 7.50 0.150
SHV-2 Ki 0.16 0.36 0.04
IC50 0.020 0.57 0.049
Vmax 100 6.5 70 1
Km 3.5 12 ND ND 24 18 NA 10
Kcat 206 ND ND 11 NA
Kcat/Km 17 ND ND 0.6 0.008
SHV-2a Ki 13 5 72 4 3 0.08 0.47 0.027
IC50 100 53 1 10 0.018 0.68 0.038
SHV-4 Vmax 100 52 115 5
Km 3.5 60 25 0.5
SHV-5 Ki 0.10 0.18 0.036
IC50 0.005 0.40 0.022
Km 15 11 3 23 7 0.02
Vmax/Km 100 100 51 4 7
SHV-7 Km 2.7 24 11 13
Vmax 35 13 30 3.3
SHV-9 Vmax 100 215 58 10 24
Km 18 12 5 18 9
IC50 0.14 0.43
SHV-13 Km 10 28 18 91 11 77
Vmax 100 178 136 0.38 12 0.66
Vmax/Km 100 64 76 0.42 11 0.86
SHV-18 Vmax 100 200 13.5 26.9 < 1
Vmax/Km 100 53 1.5 24 ND
SHV-24 Vmax 2 2.37 0.043 0.735
Km 32 210 30 500
Vmax/Km 0.0625 0.0113 0.000143 0.00147
Ki 57 ND 37 ND
SHV-38$ Kcat 100 1800 10 100 40 5 110 1 3 3
Km 13 35 14 80 150 100 3800 800 1600 5500
Kcat/Km 7700 51,000 700 1300 270 50 30 1 2 0.5
SHV-55* Km 5 ± 0.51 10 ± 0.14 6 ± 0.02 8 ± 0.37 9 ± 0.68 58 ± 7.40 21 ± 0.13 149 ± 2.61 5 ± 0.62
Kcat 23 ± 0.76 23 ± 0.17 8 ± 0.00 27 ± 1.53 38 ± 3.94 9 ± 0.21 24 ± 0.34 30 ± 3.10 < 0.1
Kcat/Km 5.3 ± 0.42 2.5 ± 0.002 1.5 ± 0.00 3.7 ± 0.03 4.4 ± 0.78 0.2 ± 0.02 1.1 ± 0.01 0.2 ± 0.02 ND
IC50 0.02
SHV-57 Km 67 30.9
Kcat 3.8 × 10−3 8.6 × 10−4
Kcat/Km 5.67 × 10−5 2.78 × 10−5
Ki 27 × 103 1.16 × 103
SHV-99* Km 12 ± 0.11 11 ± 0.26 5 ± 0.93 13 ± 1.43 102 ± 11.38 136 ± 4.09 183 ± 0.72 196 ± 0.60
Kcat 778 ± 616 563 ± 8 58 ± 2 563 ± 13 37 ± 2 < 0.1 < 0.1 0.5 ± 0.001
Kcat/Km 62.3 ± 4.4 49.6 ± 1.8 13 ± 2.4 43.5 ± 6.5 0.37 ± 0.04 < 0.001 < 0.001 0.003
IC50 0.02 0.03
SHV-104 Kcat 55 80 30 >1.8
Km 94 10 68 >600
Kcat/Km 0.6 8 0.44 0.003
SHV-129#* Kcat 22.8 ± 11 1688 ± 4 26 ± 1 3.1 ± 1.5 4.8 ± 3.4 4.5 ± 0.5
Km 46.8 ± 24 25 ± 9 12.1 ± 3.7 24 ± 3 26.7 ± 5.5 52 ± 3.5
Kcat/Km 0.5 ± 0.7 7 ± 0.4 2.2 ± 0.3 0.13 ± 0.5 0.2 ± 0.5 0.09 ± 0.01
Ki 0.4 0.4 0.04

Antibiotic: Penicillin (PEN); Ampicillin (AMP); Amoxicillin (AMX); Ticarcillin (TIC); Piperacillin (PIP); Cephaloridine (CER); Cephalothin (CEF); Ceftazidime (CAZ); Cefotaxime (CTX); Cefepime (FEP); Aztreonam (ATM); Clavulanic Acid (CLA); Sulbactam (SUL); Tazobactam (TZB).

Parameters are expressed as μM (Ki, IC50, Km), s−1(Kcat), and μM/min (Kcat/Km, Vmax). Only antibiotics for which 3 or more SHV enzyme values were available are reported.

NA, not able to determine the rate of hydrolysis and affinity; NH, not hydrolyzed; ND, not determined.

**

Non ESBL SHV-1 is provided as reference.

$

Kcat/Km values are expressed as mM/s.

#

Kcat/Km values are expressed as μM/s.

*

Values (Except IC50) represent mean ± standard deviation.

References: SHV-1 (Gutmann et al., 1989; Poirel et al., 2003); SHV-2 (Gutmann et al., 1989; Bradford et al., 1995; Winkler and Bonomo, 2016); SHV-2a (Podbielski et al., 1991); SHV-4 (Péduzzi et al., 1989); SHV-5 (Gutmann et al., 1989); SHV-7 (Bradford et al., 1995); SHV-9 (Prinarakis et al., 1996); SHV-13 (Yuan et al., 2000); SHV-18 (Rasheed et al., 2000); SHV-24 (Kurokawa et al., 2000); SHV-38 (Poirel et al., 2003); SHV-55 (Mendonça et al., 2006); SHV-57 (Ma et al., 2005): SHV-99 (Ramdani-Bouguessa et al., 2011); SHV-104 (Ben Achour et al., 2014); SHV-129 (Winkler and Bonomo, 2016).