FIGURE 1.

Sema-1a expression analysis. Anti-GFP labeling (green) of the Sema-1a^CA07125 GFP protein trap line combined with anti-FasII labeling (magenta) visualizing the α, β, and γ lobes of the MBs and the ellipsoid body (EB; Fushima and Tsujimura, 2007). Sema-1a is expressed in cell bodies throughout the cortex of the larval and adult brain as well in as axonal tracts such as the larval MBs and the adult EB and MB. We detected labeling in all the MB lobes of third instar larvae as well as during all pupal stages. (A) Wandering third instar larvae: anti-FasII labels the γ-lobes, other GFP positive MB lobes consist of the developing α′ and β′ lobes (B) 25% pupae: anti-FasII labels α, β and weakly the γ lobes, other GFP positive MB lobes consist of the developing α′ and β′ lobes. (C) 50% pupae: anti-FasII labels α, β and weakly the γ lobes, other GFP positive MB lobes consist of the developing α′ and β′ lobes. We also observe GFP expression in the MB Kenyon cells (KC; inset). (D) 75% pupae: anti-FasII labels α, β, and γ lobes, other GFP positive MB lobes consist of the developing α′ and β′ lobes.