FIGURE 1.

Identification of RD29A::TaFBA1 transgenic tobacco lines. (A) Phenotype of 35S::TaFBA1 (T3, T8), RD29A::TaFBA1 (RF-3, RF-4) and WT plants at 40, 80 days after sowing. (B) Identification of RD29A::TaFBA1 transgenic tobacco lines by genome PCR. WT, wild-type; M, DNA marker. (C) Identification of RD29A::TaFBA1 transgenic plants by real-time RT-PCR. Three independent transgenic lines (RF-3, 4, and 9) were used. (D) TaFBA1 protein abundance in transgenic plants in different drought conditions, as revealed by western blot analysis, rubisco large subunit was used as a loading control.