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. 2016 Aug 20;9:51–56. doi: 10.1016/j.dib.2016.08.012

Fig. 1.

Fig. 1

Experimental Workflow for LRM isolation and analysis. Populations of wt-CFTR and F508del-CFTR HeLa cells were labeled using SILAC and treated or not with 100 U/mL TNF-α. Extracts were mixed 1:1 and LRM were isolated with a sucrose gradient, digested in solution with trypsin and subjected to LC–MS/MS analysis.