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(a) Isolation of LRM fractions from HeLA cells. Twelve fractions (fractions 9–12 were pooled) were collected from the sucrose density gradient and analysed for marker proteins of LRM, caveolin-1 and flottilin-1, by western blot. LRM fractions are indicated. (b) Representation of proteins classified as membrane proteins in the total lysate and the LRM fraction, according to « GO cellular component slim » name « membrane ».
