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. 2016 Aug 26;9:229–243. doi: 10.1016/j.redox.2016.08.007

Fig. 5.

Fig. 5.

Quercetin protects against cholesterol-induced oxidative stress in Min6 cells. After treatment of Min6 cells for 6 h with 320 µM cholesterol and/or 1, 10, 50 or 100 µM QUE, cellular oxidative status was determined through (A) DCFD oxidation and (B) DHE oxidation. (C) Mitochondrial and oxidative status was determined using MitoSox™ Red oxidation. Oxidative stress was determined by measuring lipid oxidation by TBARS (D) in Min6 cells and (E) in pancreas. Values are expressed as mean±SEM, from three independent culture preparations, each treatment performed in quadruplicate. All one-way ANOVA (p values specified in results), symbols indicate Bonferroni post-test significances #relative to control and *to cholesterol-treated Min6 cells. MDA, malondialdehyde.