Fig. 7.

Quercetin protects against the increase in NFκB translocation to the nucleus in the pancreas from rats fed high cholesterol diet and in Min6 cells exposed to cholesterol. Nuclear extracts from A) pancreas of rats fed for 4 weeks with control diet (C), control diet containing 0.5% quercetin (CQ), high cholesterol diet (HC) and high cholesterol diet containing 0.5% quercetin (HCQ) and B) nuclear extracts from cells incubated for 6 h with 320 µM cholesterol and/or 50 µM QUE and NFκB (p65) were quantified. Values are expressed as percentage relative to positive control (HeLa cell lysate containing TNFα-activated NFκB (p65), 100%). Values are expressed as mean±SEM. N=6–8 rats/group. In the vitro study three independent culture preparations were assessed, in which each treatment was performed in quadruplicate. All two-way ANOVAs, symbols indicate Bonferroni post-test significances ^#compared to control diet and *to HC diet; or ^#relative to control and *to cholesterol-treated Min6 cells.