Table 2.
Quercetin and cholesterol modulate gene expression: in vivo and in vitro studies.
|
Pancreas | ||||
|---|---|---|---|---|
| Gene [relative fold expression] | C | CQ | HC | HCQ |
| Sirt1 | 1.00±0.07 | 1.54±0.15***,## | 0.13±0.07### | 1.51±0.12***,# |
| PGC-1α | 1.00±0.19 | 2.54±0.50**,# | 1.98±0.11# | 3.30±0.36**,### |
| PPAR γ | 1.00±0.13 | 0.30±0.02***,## | 1.68±0.21## | 1.15±0.03* |
| LXRα | 1.00±0.11 | 0.30±0.07***,# | 1.89±0.31## | 0.99±0.06** |
| Min6 cells | ||||
| Gene [relative fold expression] | Control | Quercetin 50 µM | Cholesterol 320 µM | Quercetin 50 µM + Cholesterol 320 µM |
| Sirt1 | 1.00±0.02 | 1.35±0.07***,### | 0.89±0.04# | 1.33±0.12***,### |
| PGC-1α | 1.00±0.09 | 1.66±0.18### | 1.46±0.20## | 2.38±0.09***,### |
| PPAR γ | 1.00±0.26 | 0.88±0.07*** | 1.77±0.17### | 1.37±0.03*,# |
Gene expression in plasma from rats fed control diet (C), control diet containing 0.5% quercetin (CQ), high cholesterol diet (HC) and high cholesterol diet containing 0.5% quercetin (HCQ). Values are expressed as mean ± SEM. N=6–8 rats/group.
Gene expression in Min6 cells treated for 20 h with 320 µM cholesterol and/or 50 µM QUE. Values are expressed as mean ± SEM, from three independent culture preparations, each treatment performed in quadruplicate. All two-way ANOVA, symbols indicate Bonferroni post-test significances #in comparison to control and *to (high)-cholesterol.*p<0.05, **p<0.01, ***p<0.001; #p<0.05, ##p<0.01, ###p<0.001.