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. 2016 Aug 30;57(6):1412–1419. doi: 10.3349/ymj.2016.57.6.1412

Fig. 3. Effect of NPB311 on [Ca2+] in RBL-SX38 cells. (A) RBL-SX38 cells (5×104/well) were incubated at 37℃ for 24 h. NP-IgE were treated with/without NPB311 (0, 0.16, 0.8, 4, 20, 100, and 500 nM) and incubated at 37℃ for 2 h, then FLIPR calcium 4 dye were added. After incubation for 1 h, NP-BSA was added and then FLUO-4AM (relative fluorescence units, RFU) was detected every 2 sec and 160 times. (B) RBL-SX38 cells (5×104/well) were incubated at 37℃ for 24 h. NPB311 (0 and 500 nM) were incubated 37℃ for 2 h, then FLIPR calcium 4 dye were added. After incubation, NP-BSA was added and then FLUO-4AM was detected every 2 sec and 90 times. Results are presented as mean±SD from four independent experiments. FLIPR, Fluorometric Imaging Plate Reader.

Fig. 3